Abstract: In the traditional molecular biology experiment, α-complementation method for screening recombinants and protein expression in prokaryotic cells were two independent experimental technologies. In order to make up for the lack of continuity of these two experiments,a comprehensive experiment of gene cloning and protein expression integrative in one vector was designed to strengthen the students􀆶 understanding of the reading frame. The comprehensive experimental design in molecular biology experiments could expand the span and depth of experimental teaching, and laid the foundation for further promoting students􀆶 knowledge, ability and professionalism.

Key words: molecular biology, experimental teaching, T vector, vector construction, protein expression